| 产品编号 | bs-2503R-BF488 |
| 英文名称 | Rabbit Anti-eEF1A1/BF488 Conjugated antibody |
| 中文名称 | BF488标记的翻译延伸因子EF-1a抗体 |
| 别 名 | Elongation Factor 1, Alpha; EF1 Alpha; eukaryotic translation elongation factor 1 alpha 1; CCS3; eEF1A-1; EF-Tu; EF1A; Elongation factor Tu; GRAF-1EF; PTI1; elongation factor 1-alpha 1; EEF-1; EEF1A; Eef1a2; Eef1a2l1; EF1A; MGC125172; SI; Eef1a1; EF1A1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EF1 alpha |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: eEF1A1 is an isoform of the alpha subunit of the elongation factor 1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. It promotes the GTP dependent binding of aminoacyl tRNA to the A site of ribosomes during protein biosynthesis. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (referenced from entrez gene and swissprot). Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Subcellular Location: Cytoplasm. Tissue Specificity: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle Post-translational modifications: ISGylated. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. Database links: Entrez Gene: 1915 Human Entrez Gene: 13627 Mouse Omim: 130590 Human SwissProt: P68104 Human SwissProt: P10126 Mouse Unigene: 520703 Human Unigene: 535192 Human Unigene: 586423 Human Unigene: 695681 Human Unigene: 713109 Human Unigene: 138471 Mouse Unigene: 335315 Mouse Unigene: 360075 Mouse Unigene: 391071 Mouse Unigene: 468942 Mouse Unigene: 144660 Rat Unigene: 965 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 肽链延长因子1A-1(peptide elongation factor 1A-1,eEF1A-1)是真核细胞蛋白翻译过程中肽链延长复合物的重要构成成分,具有调节蛋白合成及促发凋亡等多种作用. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
